Coding

Part:BBa_K1585300:Design

Designed by: Michael Osthege   Group: iGEM15_Aachen   (2015-08-23)


Glycogen synthase GlgA from E. coli K12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 916


Design Notes

Six illegal restriction sites were removed to ensure full BioBrick compability. The native stop codon was replaced with TAATAA.


Source

The BioBrick was created from the genomic sequence of glgA in E. coli K12.

References